Inverted Point Confocal – University of Copenhagen

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Center for Advanced Bioimaging (CAB) Denmark > Instruments > Inverted Point Confocal

Inverted Confocal

  • Inverted confocal microscope
  • Objectives: dry: 10x 20x, immersion: 40x 100x oil; 63x water
  • Spectrometric imaging with four freely selectable emission settings
  • Line and frame sequential scanning
Fura-2 recording

i) Pancreatic acinar cells loaded with acridine orange. (ii) Intracellular Calcium signals monitored with Fura-2. (iii) Human pancreatic duct cells loaded with calcein (Ivana Novak, unpubl.)

Laser lines

Beam splitters

Emission

Detectors

351, 364 nm UV

DD488/568, TD 488/568/647

freely

 

458,488,496,

514,568,647nm

TR 30/70, RSP 500

substratum

selectable emission ranges 

4 PMT