Upright point-scanning confocal

  • Objektives: 10x, 20x, 40x oil and 63x water
  • Laser: 405, 488, 555 and 639
  • 2 PMT
  • Smart set up for choosing relevant filter + PMT setup

N. benthamiana leaf epidermal cells transiently expressing constructs encoding the fusion proteins CAMTA3_NnYFP and DSC1_NcYFP. Bimolecular fluorescence complementation (BiFC): bright field, YFP and chlorophyll b auto-fluorescence (red).

Laser lines

Beam splitters

Emission

Detectors

405 variable secondary dichoic  (vsd) beamsplitter a range of SP and LP filters 2 PMT
488 variable secondary dichoic  (vsd) beamsplitter a range of SP and LP filters 2 PMT

555

variable secondary dichoic  (vsd) beamsplitter

a range of SP and LP filters

2 PMT

639

variable secondary dichoic  (vsd) beamsplitter

a range of SP and LP filters

2 PMT