High-end confocal for FLIM – University of Copenhagen

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Center for Advanced Bioimaging (CAB) Denmark > Instruments > High-end confocal for ...

High-end confocal for FLIM

  • Confocal imaging with up to 25 fps
  • Objectives: 10x, 20x, immersion: 20x, 63x water, 40x, 63x oil
  • Supercontinuum white light laser (WLL) with up to 8 excitation lines between 470nm and 670nm
  • Argon Ion laser with 5 fast lines
  • Pulsed diode laser emitting at 405 nm
  • Two-photon excitation with internal or non-descanned detectors
  • line, frame and stack sequential scanning
  • Spectrometric imaging with five freely selectable emission ranges and settings for any fluorochrome. One detector is a HyD SP for very sensitive detection
  • MP and WLL FLIM using an external PMT and filters.

Living pancreatic duct cells, calcium signal in response to ATP

Laser lines

Beam splitter

Emission

Detectors

pulsed 405 nm UV,V

fast BS

 

458,476, 488,496,514 nm

tuneable AOBS

range freely selectable

5 PMT

470-670 nm WLL

 

 

 

690-1040 nm MP

fast BS

 

2 NDD