High Content Screening – University of Copenhagen

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CAB > Instruments > High Content Screening

Robot microscope

  • Confocal imaging in 24-, 48-, 96-, 384- or 1536-well plates
  • Environmental control: temperature, CO2, humidity
  • Objectives: 20x and 60x water immersion
  • Three CCD cameras and settings (se below) to visualize DAPI, CFP, GFP, YFP, RFP
  • Dispenser unit for addition of compounds to wells
  • Laser based auto focus
  • Script-based Image and Data analysis program that can run during the experiment
HeLa

HeLa cells transiently expressing EGFP-ALG-2 (green) and localisation of ER cargo marker p58/ERGIC (red), counterstained with Hoechst to show the nuclei (blue). Jonas Marstrand La Cour, UCPH BIO, Cell & Developmental Biology

Laser lines

Primary Dichroics (beam spitters)

CCD1

CCD2

CCD3

405 nm

405/488/561/640nm

450/50

540/75

488 nm

405/488/640 nm

520/35

565/40

561 nm

405/561/640 nm

540/75

585/40

690/50

640 nm

 

565/40

600/40

690/50