The core facility Center for Advanced Bioimaging (CAB) Denmark was funded by the programme for National Research Infrastructure, based on a proposal which gathered more than 50 research groups, mostly from the University of Copenhagen, but also from Technical University of Denmark, Aarhus University and University of Southern Denmark, around two main topics:
- the interaction between the cell and its microenvironment, comprising nutrients, ions and biomolecules, neighbouring cells, and symbiotic or pathogenic organisms; and
- genome dynamics including DNA replication and repair, cell cycle control, chromatin structure and transcription, which ultimately controls intracellular signalling and developmental processes.
The center was inaugurated in June 2011 at the University of Copenhagen (Frederiksberg and North Campus), and offers an instrument park that is unique in Denmark. It permits high resolution imaging in seven dimensions:
- 1st & 2nd Dimension Superresolution (Fluorescence localisation and 3D SIM)
- 3rd Dimension High resolution in z axis and at cell surfaces (TIRF)
- 4th Dimension High spectral resolution by confocals with freely tuneable excitation and emission wavelengths from UV to infrared
- 5th Dimension High resolution in time (Spinning disk fluorescence with EM-CCD)
- 6th Dimension High resolution for identification of chemical compounds, measuring interactions and metabolite levels (FRET, FLIM) or macro- and micronutrients in single cells (Laser ablation ICP-MS and laser dissection microscope)
- 7th Dimension High statistical resolution (High Content Screening, high content analysis; HCS/HCA) robot microscope
- Deep penetration multiphoton excitation scanning microscopes , high environmental fidelity (microscope built around an environmental chamber), and whole organism imaging.
The Center offers access and training for all relevant microscopical methods including, in addition to the above mentioned front-edge instruments also CLSM, TEM, and ESEM, a biosafety level 2 cell culture lab and a histological lab for specimen preparation. The CAB received support from the National Research Infrastructure Program and is linked to the European ESFRI project Euro-BioImaging.