Instruments

CAB instruments are to be found at two locations of the University of Copenhagen:

North Campus

High-content screening spinning disc 

Robot microscope for single cell layers to image dynamics ofcell division and differentiation and to screen libraries  

Upright point-scanning confocal

Live imaging of cells and simple tissues for (co-)localization studies of fluorochromes and fluorescent protein, FRET, FRAP

High-end confocal for FLIM

Live imaging of cells, tissue and organs, protein localization, intracellular ion concentrations, FRAP, FRET, FLIM

BioStation

Visualization of processes in living cell over extended periods of time; cell cycle, cell differentiation, migration, cell-cell interaction

Whole animal imager

In vivo imaging of mice; in vivo cancer experiments with bioluminescent or fluorescent cells; disease models

Frederiksberg Campus

Plant imaged on Stellaris

High-end inverted CLSM

Extremely versatile for many applications including live imaging of cells and tissues, localisation studies, multi-dimensional imaging (3D, time series, spectral scans...) and many others.  

Super resolution microscope

Superresolution (3D-SIM, TIRF and PALM/STORM) for protein immunolocalisation and GFP-fusion proteins  

High-end point CLSM

Live imaging of cells and tissues, colocalisation studies, 4D confocal imaging (xyzt), (xyΛ2), photoactivaiton, FRET, FRAP

Fluorescence Lifetime Microscope

Interaction studies (FLIM-FRET), analysis of diffusion dynamics (FCS), separation of fluorophores with similar spectral properties

Inverted point CLSM

Live imaging of animal cells and tissues, (co-) localisation of fluorochromes and fluorescent proteins, FRET, FRAP

Spinning disk confocal

Live imaging of ion and pH homeostasis, fluorescence cinematography, 4D confocal imaging (xyzt), FRET, FRAP

Histology laboratory

Preparation of fresh and fixed material, histology and immunofluorescence for light, fluorescence and electron microscopy  

Research microscopes

Two dissection and six wide fields microscopes allowing detection of fluorescent reporters and dyes  

Scanning electron microscope

High resolution imaging of the surface of cells, tissues and organs for fixed/goldsputtered specimens but also for low vacuum ESEM  

High resolution TEM

Negative contrast (proteins, nucleic acid or virus) and ultrathin sections of fixed bacteria or eukaryotes and immunolocalisation

Raman microscope

Identification and visualization of the distribution of chemical compounds within samples.

Infrared microscope

Mapping of chemical differences within biomass such as wood or fibres (coatings, biodegradation)

Laser ablation ICP-MS

Ablation from soft tissues; multi-elemental bioimaging; multi-plexed quantitative proteomics using lanthanide tagged antibodies

Laser microdissection

Collection of genetic and metabolite contents from single cells or small cell groups for  RT-PCR and mass spectrometry