Inverted Spinning Disk Confocal
New state-of-the-art 3i CSU-W1 SoRa Spinning Disk Confocal Microscope for high speed, high sensitivity confocal imaging.
As a special feature, this microscope is equipped with an vertical stage (optional), where, for instance, long-term imaging experiments of plants growing in the correct orientation can be carried out. A lamp for illuminating plant samples in long-term experiments is present as well, and its control including day/night cycles is integrated into the imaging software.
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Confocal imaging at widefield speed
- Objectives: 10x/0.3, 20x/0.75, 40x/0.95, 60x/1.2, 60x/1.49, 100x/1.49
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Two EM-CCD cameras for simultaneous image acquisition
- Photomanipulation/FRAP via millisecond-switching to point scanner for targeted bleaching
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Extremely customizable experiment setup with 3i Slidebook software
Laser lines | Filters |
405 nm |
445/45 |
445 nm |
482/35 |
488 nm |
525/30 |
514 nm |
542/27 |
561 nm |
617/73 |
Multiband filters: 440/521/607/700, 485/537/627
Dichroics: 405/488/561/640, 445/515/561
Animation: Daniel von Wangenheim, 3i. Image material: Isabella Østerlund, Felix Ruhnow
Details
Microscope
3i CSU-W1 SoRa inverted spinning disk confocal microscope.
Location
Frederiksberg Campus, CAB, Thorvaldsensvej 40, stair 10, 1. floor, room R164D
Typical applications
Fast and sensitive microscope great for many applications including imaging of fast kinetics, FRAP, long-term live cell imaging, multi-dimensional imaging.
Consultation
Sebastian Kjeldgaard-Nintemann
Training and problem reporting
Sebastian Kjeldgaard-Nintemann
Booking
Online via PPMS.