Inverted Point CLSM – University of Copenhagen

Confocal laser scanning microscope

  • Inverted confocal with  temperature and CO2 control
  • Objectives: 10x, immersion: 20x 63x water, 40x 100x oil
  • 405 nm violet excitation
  • line, frame and stack sequential scanning
  • Spectrometric imaging with five freely selectable emission ranges and settings for any fluorochrome

Arabidopsis root showing colocalisation of a membrane protein and a ER marker (Bangjun Wang, Markus Geisler, Alexander Schulz, unpubl).

Laser lines

Beam splitter

Emission

Detectors

405 nm UV,V

freely

 

 

458,476,488,496,514 nm

freely tuneable AOBS

 freely selectable range

5 PMTs

 543, 633 nm

freely tuneable AOBS