High-end confocal for FLIM
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Confocal imaging with up to 25 fps
- Objectives: 10x, 20x, immersion: 20x, 63x water, 40x, 63x oil
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Supercontinuum white light laser (WLL) with up to 8 excitation lines between 470nm and 670nm
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Argon Ion laser with 5 fast lines
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Pulsed diode laser emitting at 405 nm
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Two-photon excitation with internal or non-descanned detectors
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line, frame and stack sequential scanning
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Spectrometric imaging with five freely selectable emission ranges and settings for any fluorochrome. One detector is a HyD SP for very sensitive detection
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MP and WLL FLIM using an external PMT and filters.
Laser lines |
Beam splitter |
Emission |
Detectors |
pulsed 405 nm UV,V |
fast BS |
|
|
458,476, 488,496,514 nm |
tuneable AOBS |
range freely selectable |
5 PMT |
470-670 nm WLL |
|
|
|
690-1040 nm MP |
fast BS |
2 NDD |
Details
Microscope
Point-scanning Confocal and Multiphoton Microscope SP5-X MP, Leica Microsystems
Location
North Campus, August Krogh Building,
Universitetsparken 13, 6. Floor, room 644
Typical applications
Live imaging of cells, tissue and organs, protein localization, intracellular ion concentrations, FRAP, FRET, FLIM
Consultation
Nynne Meyn Christensen
Ivana Novak
Training and problem reporting
Booking
online via PPMS.