New inverted spinning disk

New spinning disk confocal microscope at CAB

The inverted 3i spinning disk confocal microscope is now installed at CAB Frederiksberg campus, a new addition granted by the CAB coordinator Staffan Persson.

Spinning disk confocal microscopes parallelize the confocal light path by placing many pinholes on a rapidly spinning disk, illuminating a camera sensor instead of a single detector. Thereby, the instrument does not require scanning, as in a confocal laser scanning microscope, and essentially allows true confocal imaging at widefield speed and with high sensitivity.  

The instrument features two very sensitive EMCCD cameras (1024x1024), 5 laser lines (405, 445, 488, 514 and 561) and a Nikon Ti2-E inverted microscope with the Yokogawa CSU-W1 SoRa spinning disk unit.

As a special feature, the 3i is equipped with both a normal horizontal stage and a vertical stage. This allows you to image, for instance, plant roots growing in their natural orientation. Coupled with a programmable light source that simulates day/night cycles, the possibility for environmental control of the stage, and a feeding mechanism for immersion medium to the objective, longtime imaging under relevant conditions can be obtained. In addition, it is possible to increase resolution up to 1.4x above the diffraction limit with the SoRa super resolution technique.

If you want to test the new instrument and see what it can do for your research, please contact Sebastian (seni@plen.ku.dk) or Nynne (nmchristensen@bio.ku.dk).